Translational_Unit

Part:BBa_K917007:Design

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-08-10)


S. oneidensis decaheme cytochrome c electron export system mtrCAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 275


Design Notes

Genes were obtained by PCR amplification of S. oneidensis MR-1 gene cluster. MtrA and MtrC contained PstI restriction sites that had to be removed by site-directed mutagensis


Source

Shewanella oneidensis MR-1 chromosomal DNA

References